Journal: Cell

Article Title: Nuclear Pores Promote Lethal Prostate Cancer by Increasing POM121-Driven E2F1, MYC, and AR Nuclear Import

doi: 10.1016/j.cell.2018.07.015

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse monoclonal anti-karyopherin a2 (B-9) , Santa Cruz Biotechnology , SC-55538; RRID: AB_831493.

Techniques: Control, Electron Microscopy, Virus, Recombinant, Extraction, shRNA, Mutagenesis, Cloning, Negative Control, Plasmid Preparation, Binding Assay, Retroviral, Luciferase, Software

Journal: Frontiers in Immunology

Article Title: Identification of Secretory Leukoprotease Inhibitor As an Endogenous Negative Regulator in Allergic Effector Cells

doi: 10.3389/fimmu.2017.01538

Figure Lengend Snippet: The depletion of basophil secretory leukoprotease inhibitor (SLPI) exacerbates IgE-mediated allergic responses. (A) The experimental protocol of IgE-mediated chronic allergic inflammation in 5-fluorouracil (5-FU)-treated Fcer1g −/− mice adaptively transferred with B6 and Slpi −/− DX5 + cells containing basophils from bone marrow (BM) cells. (B) The kinetics of the ear thickness after the antigen challenge are shown. (C) Ear specimens obtained 6 days after the antigen challenge were stained with HE. Data are representative of three separate experiments and are shown as the mean ± SD. n = 4–6. * P < 0.05, ** P < 0.01.

Article Snippet: On Day 12, c-kit − DX5 + cells were isolated as basophils using the MACS system with magnetic microbead-conjugated anti-DX5 antibody (Miltenyi Biotec).

Techniques: Staining

Journal: Arthritis Research & Therapy

Article Title: Overexpression of TLR2 and TLR9 on monocyte subsets of active rheumatoid arthritis patients contributes to enhance responsiveness to TLR agonists

doi: 10.1186/s13075-015-0901-1

Figure Lengend Snippet: Intracellular cytokine expression in monocyte subsets of rheumatoid arthritis (RA) patients after stimulation with toll-like receptor (TLR2) agonists. Mononuclear cells from RA patients and healthy donors were enriched and stimulated with lipoteichoic acid ( LTA ) ( a ) and Epstein–Barr virus ( EBV ) particles (TLR2 ligands) ( b ) in vitro. After a 5-hour stimulation, the stimulated cells were harvested and cytokine expression levels were assessed by intracellular flow cytometry. Briefly, cells were stained with CD45, CD91, CD14 and CD16, permeabilized and intracellular staining was performed on proinflammatory cytokines: IL-6, IL-1β, TNFα and monocyte chemoattractant protein-1 ( MCP-1 ). Intracellular cytokine expression is shown for classical CD14 ++ CD16 − , intermediate CD14 + CD16 + and nonclassical CD14 low CD16 ++ monocyte subsets. Gray non-stimulated samples, Blue healthy donors, red RA patients. Representative cytometry histograms are representative of ten donors

Article Snippet: Cells were stained with CD45-PerCP-Cy5.5 (2D1) (eBioscience, San Diego, CA, USA), CD91-FITC (A2-MR-α2), CD14-PE-Cy7 (M5E2) and CD16-A700 (3G8) (BD Biosciences) to discriminate between monocyte subsets, as detailed in the standardized flow cytometry assay [ ].

Techniques: Expressing, In Vitro, Flow Cytometry, Staining, Cytometry